Jennifer Holoubek is from Aurora, Illinois and began dancing just before the age of three. She received her training from Regina and Ricardo Moyano at Dance West Ballet in Naperville,Illinois. Other influential instructors included Catalina Garza Quenedit and Ernesto Quenedit. Holoubek joined Montgomery Ballet in 2012 and performed roles such as Queen of the Dryads in full-length Don Quixote, The Fairy Doll Pas de Trois, and Madame Giry in The Phantom of the Opera. She joined Ballet Tucson in 2014 and her repertory has included Snow Queen in The Nutcracker, and the Principal Russian Girl and Dark Angel in Balanchine's Serenade. Additionally, she has also appeared as a guest with Maywood Fine Arts Association performing Odette in Swan Lake. 

In addition to performing, Holoubek has a passion for choreography. At the age of twelve, she began choreographing for Regional Dance America's Midstates Festivals in Illinois. Her piece, The Dream was selected to be performed on the emerging night of performances. At sixteen she was a recipient of the Monticello Award and attended Craft of Choreography on full scholarship as an emerging choreographer. Since joining Ballet Tucson, she has choreographed several pieces for the Footprints at the Fox show, winning second place in 2017 for her piece Rakhma and first place in 2019 for La Terre vue du ciel. She is currently working towards a Performing Arts Degree with the LEAP program through Saint Mary's College of California.